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Consultar: Programa de Ps-Graduao em Cincias Farmacuticas

Incio > Dissertaes e Teses > Cincias da Sade > Farmcia > Programa de Ps-Graduao em Cincias Farmacuticas

Ttulo [PT]: Atividade antifngica de Stryphnodendron adstringens (Mart.) Coville sobre leveduras de isolados clnicos
Autor(es): Kelly Ishida
Palavras-chave [PT]:

Stryphnodendron adstringens. Atividade antifngica. Taninos. Agente antifngico. Barbatimo. Ensaio biolgico. Candida albicans. Brasil.
Palavras-chave [EN]:
Antifungal. Barbatimo. Candida sp. Stryphnodendron adstringens. Yeast. Brazil.
rea de concentrao: Produtos Naturais e Sintticos Biologicamente Ativos
Titulao: Mestre em Cincias Farmacuticas
Banca:
Celso Vataru Nakamura [Orientador] - UEM
Resumo:
Resumo: Em recursos naturais, principalmente plantas, tm se buscado molculas bioativas que podem se tornar uma alternativa para o desenvolvimento de novos agentes antifngicos com menos efeitos colaterais, amplo espectro de ao e menor custo. Assim, o objetivo deste trabalho foi verificar o efeito do extrato bruto, fraes e subfraes de Stryphnodendron cidstringens (Mart.) Covilie, popularmente conhecido como "barbatimo", sobre leveduras isoladas de fluido vaginal. Amostras de fluido vaginal foram coletadas de 506 pacientes ambulatoriais, sendo que 103 apresentaram cultura positiva para leveduras (20,35%). Dentre essas, 96/103 (93,2%) foram identificadas como Candida albicans, 6 como C. no albicans (1 C. parapsilosis, 1 C. tropicalis e 4 Candida sp.) e uma amostra como no pertencente ao gnero Candida. Todas as amostras foram susceptveis aos polinicos, e somente 7,76 % resistentes ou susceptvel dose dependente ao fluconazol. A maioria das amostras produziram protease cida (99%), fosfolipase (83%) e hemolisina (9 1,3%). A aderncia de leveduras em clulas Vero foi variada, sendo que 67,9% das amostras apresentaram aderncia de 50 a 1 50%, e a maioria possuem caractersticas hidrofihicas. As amostras foram susceptveis aos extratos de S. adsiringens em concentraes superiores a 7,8 g/mL mostrando somente atividade fungisttica. A subfrao F2.4 do "barbatimo" inibiu a formao do tubo germinativo em concentraes ≥15,75 g/mL, e um aumento significativo de brotamentos (188,34%) foi observado em leveduras tratadas com 125 g/mL. A inibio da aderncia de leveduras em clulas e superfcie abitica foi dose dependente. A subfrao F2.4 reduziu a hidrofobicidade de superfcie celular. Atravs da metodologia empregada nesse estudo, no foi possvel detectar a inibio da atividade de enzimas extracelulares de Candida spp. Interaes da subfrao F2.4 com anfotericina B, fluconazol e nistatina no foram sinrgicas. Atravs da microscopia ptica e de varredura pde-se observar a inibio na formao de pseudohifas e efeito aglutinante da subfrao F2.4. Irregularidades na parede celular de Ieveduras tratadas com a subfrao F2.4 tambm foram visualizadas, e a microscopia eletrnica de transmisso evidenciou o efeito sobre a parede celular. Adicionalmente, a estimulao macrofgica foi verificada quando as leveduras eram previamente tratadas com 7%8 g/mL da subfrao F2.4 por 2 h, observando um aumento de 28% em relao ao controle. A citotoxicidade dos extratos do "barbatimo" foi observada sobre clulas Vero e macrfagos com CC50 acima de 100 g/mL. Por outro lado, para hemcias e larvas de Artemia salina a CC50 e DL50 foram superiores a 1000 g/mL, exceto para a subfrao F2.4 com DL50 de 100 g/mL. Provavelmente a atividade antifngica da subfrao F2.4 observada neste trabalho se atribui s proantocianidinas polimricas triidroxilados no anel B e presena dc cido glico na sua estrutura, com estimativa de peso molecular mdio de 2.000 Da.

Abstract: In natural products, mainly plants, have searched bioactivies compounds which can become an alternative for development of news antifungal agents with fewer side effects, a greater spectrum of action and Low cost. So, the aim of this study was verify the effect of rude extract, fractions, and sub-fractions otained from Stryphnodendron adstringens (Mart.) Coville, known as "barbatimo", on yeasts isolated from vaginal fluid. Of 506 vaginal fluid sampIes, 103 had positive culture on dextrose Sabouraud gar (20.35%). Ninety and six sampIes were identified as Candida albicans and, six C. non- albicans (1 C. parapsilosis, 1 C. tropicalis and 4 Candida sp). One no concern genus Candida. All yeasts were susceptible to polyenes and, just 7.76% were resistants or dose dependent susceptible. The most of yeast produced acid protease (99%), phospholipase (83%) and hemolysin (91.3%). Adherence on Vero cells was varied, being 67.9% with 50 to 150 yeasts/100 cells, and the most had hydrophilics characteristics. The extracts of S. adstringens showed antifungal activity in concentration above 7.8 g/mL, however just fungistatic action. The sub-fraction F2.4 inhibited the germinative tube formation in concentrations 15.75 In natural products, mainly plants, have searched bioactivies compounds which can hconic an alternative for development of news antifungal agents with fewer side effects, a ienter spectrum of action and Low cost. So, the aim of this study was verify the effect of rude extract, fractions, and sub-fractions obtained from Stryphnodendron adstringens (Mart.) Coville, known as "barbatirno", on yeasts isolated from vaginal fluid. Of 506 vaginal fluid umpIes, 103 had positive culture on dextrose Sabouraud gar (20.35%). Ninety and six anipIes were identified as Candida albicans and, six C. non- albicans (1 C. parapsilosis, 1 C. fropicalis and 4 Candida sp). One no concern genus Candida. All yeasts were susceptible to polycncs and, just 7.76% were resistants or dose dependent susceptible. The most of yeast produced acid protease (99%), phospholipase (83%) and hemoLysin (91.3%). Adherence on Vem edis was varied, being 67.9% with 50 to 150 yeasts/100 cells, and the most had hydrophilics characteristics. The extracts of 8. adstringens showed antifungal activity in onccntration above 7.8 g/mL, however just fungistatic action. The sub-fraction F2.4 inhibited the germinative tube formation in concentrations 15.75 g/mL and significative ncrcase of buddings (188.34%) was observed in yeasts treated with 125 g/mL. Adherence inhibition of yeast on cell and a biotic surface was dependent dose. The sub-fraction F2.4 reduced the cellular surface hydrophobicity. In this study was not possible verify the inhibition of extracellular enzymes activities. Combinations of sub-fraction F2.4 with amphotericin B, nystatin or fluconazole were not synergic. Through optic and scanning cletronic microscopy can be observed the inhibition in pseudohyphal formation and aggiutinant effect of sub-fraction F2.4. Irregularity on cell wall of yeasts treated was observed and transrnission eietronic microscopy showed evidence of the effect of "barbatimo" on yeast wall cdl. Additionally, the macrophage stimulation was verify when the yeasts were tu(ccI previously with 7.8 g/mL of sub-fraction F2.4 by 2 h, observing increase of 28% iimpared with control group. Cytotoxicity effect ofextracts obtained from "barbatirno" was hNeaved on Vero and rnacrophages celis with CC50 above 100 g/mL. On the other hand, for hlood red ccli and Aflemia salina grub the CC50 and DL50 were >1,000 g/mL, except for subl (ion F2.4, which DL50 was 100 jig/mL. Probably the antifungal activity of sub-fraction 1 /4 is attributed the polyrners proanthocyanidins tri-hydroxylated in ring B and presence of acid Iinked in structure ofpolymer, with median molecular weight of 2,000 15.75 g/mL and significative ncrcase of buddings (188.34%) was observed in yeasts treated with 125 g/mL. Adherence inhibition of yeast on cell and a biotic surface was dependent dose. The sub-fraction F2.4 reduced the cellular surface hydrophobicity. In this study was not possible verify the inhibition of extracellular enzymes activities. Combinations of sub-fraction F2.4 with amphotericin B, nystatin or fluconazole were not synergic. Through optic and scanning cletronic microscopy can be observed the inhibition in pseudohyphal formation and aggiutinant effect of sub-fraction F2.4. Irregularity on cell wall of yeasts treated was observed and transrnission eietronic microscopy showed evidence of the effect of "barbatimo" on yeast wall cdl. Additionally, the macrophage stimulation was verify when the yeasts were tu(ccI previously with 7.8 g/mL of sub-fraction F2.4 by 2 h, observing increase of 28% iimpared with control group. Cytotoxicity effect ofextracts obtained from "barbatirno" was hNeaved on Vero and rnacrophages celis with CC50 above 100 jig/mL. On the other hand, for hlood red ccli and Aflemia salina grub the CC50 and DL50 were >1,000 g/mL, except for sub-fraction F2.4, which DL50 was 100 g/mL. Probably the antifungal activity of sub-fraction 1 /4 is attributed the polyrners proanthocyanidins tri-hydroxylated in ring B and presence of acid Iinked in structure ofpolymer, with median molecular weight of 2,000 Da.
Data da defesa: 2006
Cdigo: vtls000164660
Informaes adicionais:
Idioma: Portugus
Data de Publicao: 2006
Local de Publicao: Maring, PR
Orientador: Prof. Dr. Celso Vataru Nakamura
Co-Orientador: Prof. Dr. Benedito Prado Dias Filho
Instituio: Universidade Estadual de Maring. Departamento de Farmcia e Farmacologia
Nvel: Dissertao (mestrado em Cincias Farmacuticas)
UEM: Programa de Ps-Graduao em Cincias Farmacuticas

Responsavel: elaine
Categoria: Aplicao
Formato: Documento PDF
Arquivo: Dissertao Kelly Ishida.pdf
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Criado: 17-06-2016 13:58
Atualizado: 17-06-2016 14:24
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